Cattle F2(Coagulation Factor II) ELISA Kit

Description: A sandwich ELISA kit for detection of Coagulation Factor II from Cattle in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Description: serum.

Description: A sandwich quantitative ELISA assay kit for detection of Rat Coagulation Factor II (F2) in samples from serum.

Description: A sandwich quantitative ELISA assay kit for detection of Rat Coagulation Factor II (F2) in samples from serum.

Description: serum.

Description: Pig (Sus scrofa; Porcine)

Description: Pig (Sus scrofa; Porcine)

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: serum.

Description: serum.