General PCr(Phosphocreatine) ELISA Kit

Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of General Phosphocreatine (PCr) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PCr protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PCr. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PCr in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PCr protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PCr. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PCr in the samples is then determined by comparing the OD of the samples to the standard curve.