General SB(Stercobilin) ELISA Kit

Description: A competitive Inhibition ELISA kit for detection of Stercobilin from General in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SB in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SB in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: 17095-63-5