Isotretinoina orale e uso topico di retinoidi in una serie di giovani pazienti con melanoma oculare

Scopo: descrivere la prima serie di sei pazienti giovani con melanoma uveale (UM) con isotretinoina orale e / o terapia topica con retinoidi prima della diagnosi.

Osservazioni: la serie di casi si basa su osservazioni cliniche presso il nostro centro di riferimento quaternario UM. Vengono segnalati sei casi di pazienti UM, di età compresa tra 16 e 44 anni. Tutti avevano usato un trattamento con retinoidi orale (isotretinoina) e / o topico (tretinoina o tazarotene) (3 mesi- ~ 10 anni) prima o al momento della diagnosi (3 su 6 casi). Tutti i pazienti presentavano disturbi oculari e l’insorgenza di alcuni sintomi corrispondeva al decorso dei retinoidi. Altri potenziali fattori di rischio o anamnesi rilevante includevano sfondo caucasico, distrofia conica e stato di fumatore attivo (caso 2), storia familiare di UM e gravidanza al momento della diagnosi (caso 3), fumo passato e possibile esposizione secondaria a Chernobyl da bambino ( Caso 5). Tutti i pazienti sono stati trattati con radioterapia a fascio di protoni e attualmente non hanno segni di malattia recidivante o metastatica.

Conclusioni e importanza: la terapia con retinoidi è stata collegata a vari effetti benigni e / o reversibili sull’occhio anteriore e posteriore, sebbene la fisiopatologia non sia ancora ben compresa. Il melanoma uveale (UM) è una rara diagnosi di cancro nei giovani adulti. Riportiamo qui la prima serie di casi di giovani pazienti di UM con una storia di uso di retinoidi e disturbi oculari. Non viene affermato alcun nesso causale e ulteriori studi epidemiologici e biologici sistematici sulla terapia con retinoidi e sull’impatto oculare possono fornire ulteriori dati rilevanti, in particolare nei pazienti giovani con melanoma oculare.

Aggiornamento teratogeno: uso topico e retinoidi di terza generazione

I primi retinoidi farmaceutici approvati dalla Food and Drug Administration degli Stati Uniti hanno ricevuto avvertimenti da scatola nera contro il loro uso in gravidanza a causa di potenziali effetti teratogeni. Questi retinoidi di prima e seconda generazione sono stati inizialmente formulati per il dosaggio orale e sono strutturalmente molto simili alla vitamina A, che ha effetti benefici sulla pelle e svolge un ruolo fondamentale nel guidare l’embriogenesi sana. Da allora, alcuni di questi retinoidi precoci sono stati riformulati per l’applicazione topica, il che ha ridotto il loro potenziale di esposizione sistemica. Inoltre, la progettazione razionale dei farmaci è stata applicata per creare i retinoidi di terza generazione di oggi (adapalene, tazarotene e bexarotene).

Questi composti includono anelli aromatici all’interno dei loro nuclei molecolari per fornire una rigidità strutturale che contrasta con la spina dorsale alifatica flessibile della vitamina A e delle precedenti generazioni di retinoidi, e quindi limita la loro attività fuori bersaglio. Come risultato di queste caratteristiche progettuali, il potenziale teratogeno negli animali dei retinoidi di terza generazione e in quelli riformulati per uso topico è generalmente inferiore a quello osservato con la somministrazione orale di generazioni precedenti di retinoidi. I dati epidemiologici disponibili, ma limitati, mostrano inoltre un potenziale teratogeno minimo o nullo associato all’uso nella vita reale di questi composti nell’uomo. Data la scarsità di dati epidemiologici disponibili in questo momento, tuttavia, si raccomanda di evitare l’uso di retinoidi topici durante la gravidanza. Tuttavia, in circostanze in cui può verificarsi un’esposizione involontaria in gravidanza, i dati disponibili forniscono una certa rassicurazione sul fatto che gli esiti avversi della gravidanza sono improbabili.

Chemerin come un driver di ipertensione: una considerazione

La proteina chemerina (gene indotto dal tazarotene, TIG2; RARRES2) è un’adipochina relativamente nuova. Molti studi supportano che i livelli di chemerina circolanti si associano fortemente e positivamente con BMI, grasso viscerale e pressione sanguigna. Qui, ci concentriamo sulla relazione specifica tra chemerina e pressione sanguigna con l’obiettivo di capire se e come la chemerina guida i cambiamenti (patologici) della pressione sanguigna in modo tale da poter essere interferiti terapeuticamente. Analizziamo la biosintesi della chemerina e come gli attuali farmaci antipertensivi modificano il metabolismo delle chemerine. Segue una rassegna di ciò che è noto su dove la chemerina è sintetizzata nel corpo e cosa possono fare la chemerina ei suoi recettori per la funzione fisiologica degli organi importanti per la determinazione della pressione sanguigna (ad es. Cervello, cuore, reni, vasi sanguigni, surrene e sistema nervoso simpatico).

Sintetizziamo dalla letteratura la nostra migliore comprensione dei meccanismi con cui la chemerina modifica la pressione sanguigna, con la consapevolezza che i livelli plasmatici / sierici di chemerina possono essere limitati nella loro rilevanza patologica. Questa revisione rivela diverse lacune nella nostra conoscenza della biologia delle chemerine che potrebbero essere colmate dal lavoro collettivo di chimici delle proteine, biologi, farmacologi e medici. Il tazarotene è un profarmaco che appartiene alla classe acetilenica dei retinoidi. Il farmaco è stato sottoposto a stress test idrolitico, ossidativo e fotolitico per stabilire la sua chimica di degradazione completa. Il farmaco si è dimostrato instabile in condizioni idrolitiche acide e basiche, producendo acido tazarotenico, che è un noto prodotto di degradazione principale (DP) e un metabolita attivo.

Soft Tissue Tissue Slide (Skeletal Muscle-Benign)
12-343-10um	ProSci	10 um	241.8 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-Benign)
12-343-4um	ProSci	4 um	216.6 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-Normal)
MBS154227-10um	MyBiosource	10um	210 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-Normal)
MBS154227-4um	MyBiosource	4um	195 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-Normal)
MBS154227-5x10um	MyBiosource	5x10um	920 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-Benign)
MBS154290-10um	MyBiosource	10um	210 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-Benign)
MBS154290-4um	MyBiosource	4um	195 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-Benign)
MBS154290-5x10um	MyBiosource	5x10um	920 EUR
Rat Skeletal Muscle Tissue Lysate
IRTSKMTL100UG	Innovative research	each	245 EUR
Description: Rat Skeletal Muscle Tissue Lysate
Rat Skeletal Muscle Tissue Lysate
LYSATE0010	BosterBio	200ug	180 EUR
Description: This cell lysate is prepared from rat skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Rat Skeletal Muscle Tissue Lysate
MBS8421547-01mg	MyBiosource	0.1mg	385 EUR
Rat Skeletal Muscle Tissue Lysate
MBS8421547-5x01mg	MyBiosource	5x0.1mg	1540 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-malignant)
12-345-10um	ProSci	10 um	241.8 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-malignant)
12-345-4um	ProSci	4 um	216.6 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-malignant)
MBS154272-10um	MyBiosource	10um	210 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-malignant)
MBS154272-4um	MyBiosource	4um	195 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-malignant)
MBS154272-5x10um	MyBiosource	5x10um	920 EUR
Human Skeletal Muscle Tissue Lysate
IHUSKMTL100UG	Innovative research	each	245 EUR
Description: Human Skeletal Muscle Tissue Lysate
Mouse Skeletal Muscle Tissue Lysate
IMSSKMTL100UG	Innovative research	each	245 EUR
Description: Mouse Skeletal Muscle Tissue Lysate
Mouse Skeletal Muscle Tissue Lysate
LYSATE0021	BosterBio	200ug	180 EUR
Description: This cell lysate is prepared from mouse skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Mouse Skeletal Muscle Tissue Lysate
MBS8420876-01mg	MyBiosource	0.1mg	385 EUR
Mouse Skeletal Muscle Tissue Lysate
MBS8420876-5x01mg	MyBiosource	5x0.1mg	1540 EUR
Human Skeletal Muscle Tissue Lysate
MBS8419499-01mg	MyBiosource	0.1mg	385 EUR
Human Skeletal Muscle Tissue Lysate
MBS8419499-5x01mg	MyBiosource	5x0.1mg	1540 EUR
Bovine Skeletal Muscle Tissue Lysate
IBOSKMTL100UG	Innovative research	each	541 EUR
Description: Bovine Skeletal Muscle Tissue Lysate
Bovine Skeletal Muscle Tissue Lysate
MBS136285-01mg	MyBiosource	0.1mg	745 EUR
Bovine Skeletal Muscle Tissue Lysate
MBS136285-5x01mg	MyBiosource	5x0.1mg	3165 EUR
41225-2 RB MUSCLE TISSUE YG (1-kg)
41225-2	Pel-Freez	1 kg	225 EUR
41325-2 RB MUSCLE TISSUE MT FROZEN 1kg
41325-2	Pel-Freez	1 kg	225 EUR
Rat Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells
9-80281	CHI Scientific	1 x 100 ml	Ask for price
Human Diabetes Skeletal Muscle Tissue Lysate
IHUSKMDIBTL100UG	Innovative research	each	1413 EUR
Description: Human Diabetes Skeletal Muscle Tissue Lysate
Human Diabetes Skeletal Muscle Tissue Lysate
MBS8419496-01mg	MyBiosource	0.1mg	1810 EUR
Human Diabetes Skeletal Muscle Tissue Lysate
MBS8419496-5x01mg	MyBiosource	5x0.1mg	7965 EUR
Smooth muscle and striated muscle disease spectrum tissue array
SO8011	TissueArray	each	414 EUR
Description: Smooth muscle and striated muscle disease spectrum tissue array, including TNM and clinical stage, 80 cases /80 cores
Human Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells
9-80092	CHI Scientific	1 x 100 ml	Ask for price
Mouse Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells
9-80189	CHI Scientific	1 x 100 ml	Ask for price
Rat Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells
9-80280	CHI Scientific	1 x 100 ml	Ask for price
Human Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells
9-80091	CHI Scientific	1 x 100 ml	Ask for price
Mouse Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells
9-80188	CHI Scientific	1 x 100 ml	Ask for price
Monkey Cynomolgus Skeletal Muscle Tissue Lysate
IMNCYSKMTL100UG	Innovative research	each	541 EUR
Description: Monkey Cynomolgus Skeletal Muscle Tissue Lysate
Monkey Cynomolgus Skeletal Muscle Tissue Lysate
MBS8420209-INQUIRE	MyBiosource	INQUIRE	Ask for price
Frozen Tissue Section - Human Diabetic Diseased Tissue: Skeletal Muscle
T1236171Dia	Biochain	5 slides	523 EUR
cDNA - Dog Normal Tissue: Skeletal Muscle
C1734171	Biochain	40 reactions	424 EUR
cDNA - Rat Normal Tissue: Skeletal Muscle
C1434171	Biochain	40 reactions	424 EUR
Rhabdomyosarcoma with striated muscle tissue array
SO2082b	TissueArray	each	546 EUR
Description: Rhabdomyosarcoma with striated muscle tissue array, including TNM and clinical stage, 104 cases/208 cores, replacing SO2082a
Muscle disease spectrum (smooth muscle and striated muscle cancer progression) tissue array
SO2081	TissueArray	each	474 EUR
Description: Muscle disease spectrum (smooth muscle and striated muscle cancer progression) tissue array, 104 cases/208 cores
cDNA - Human Tumor Tissue: Skeletal Muscle
C1235171-10	Biochain	10 reactions	301 EUR
Rat Skeletal Muscle Whole tissue lysate
RAL-1467	Alpha Diagnostics	1 mg	628.8 EUR
Paraffin Tissue Section - Human Diabetic Diseased Tissue: Skeletal Muscle
T2236171Dia	Biochain	5 slides	262 EUR
cDNA - Mouse Normal Tissue: Skeletal Muscle
C1334171	Biochain	40 reactions	424 EUR
Human Skeletal Muscle Fetal Tissue Lysate
IHUSKMFSTL100UG	Innovative research	each	541 EUR
Description: Human Skeletal Muscle Fetal Tissue Lysate
Mouse Skeletal Muscle Whole tissue lysate
MAL-1407	Alpha Diagnostics	1 mg	628.8 EUR
Rat Muscle OptiTDS1: Tissue Dissociation System
4-28280	CHI Scientific	1 Kit	Ask for price
Rat Muscle OptiTDS2: Tissue Dissociation System
4-28281	CHI Scientific	1 Kit	Ask for price
Total RNA - Rat Normal Tissue: Skeletal Muscle
R1434171-50	Biochain	50 ug	156 EUR
Human Muscle OptiTDS1: Tissue Dissociation System
4-28091	CHI Scientific	1 Kit	Ask for price
Human Muscle OptiTDS2: Tissue Dissociation System
4-28092	CHI Scientific	1 Kit	Ask for price
Mouse Muscle OptiTDS1: Tissue Dissociation System
4-28188	CHI Scientific	1 Kit	Ask for price
Mouse Muscle OptiTDS2: Tissue Dissociation System
4-28189	CHI Scientific	1 Kit	Ask for price
Genomic DNA - Rat Normal Tissue: Skeletal Muscle
D1434171	Biochain	100 ug	329 EUR
Total RNA - Mouse Normal Tissue: Skeletal Muscle
R1334171-50	Biochain	50 ug	156 EUR
cDNA - Human Adult Normal Tissue: Skeletal Muscle
C1234171	Biochain	40 reactions	424 EUR
Genomic DNA - Mouse Normal Tissue: Skeletal Muscle
D1334171	Biochain	100 ug	329 EUR
cDNA - Monkey (Rhesus) Normal Tissue: Skeletal Muscle
C1534171	Biochain	40 reactions	481 EUR
Total Protein - Human Tumor Tissue: Skeletal Muscle
P1235171	Biochain	1 mg	357 EUR
Tissue, Total Protein, Human Tumor, Skeletal Muscle
MBS657457-1mg	MyBiosource	1mg	680 EUR
Tissue, Total Protein, Human Tumor, Skeletal Muscle
MBS657457-5x1mg	MyBiosource	5x1mg	2835 EUR
Total Protein - Mouse Normal Tissue: Skeletal Muscle
P1334171	Biochain	1 mg	216 EUR
cDNA - Human Diabetic Diseased Tissue: Skeletal Muscle
C1236171Dia	Biochain	40 reactions	802 EUR
cDNA - Monkey (Cynomolgus) Normal Tissue: Skeletal Muscle
C1534171-Cy	Biochain	40 reactions	481 EUR
Human Liver Cirrhosis Muscle Diaphragm Tissue Lysate
IHUMSCDPHCIRTL100UG	Innovative research	each	1413 EUR
Description: Human Liver Cirrhosis Muscle Diaphragm Tissue Lysate
Human Liver Cirrhosis Muscle Diaphragm Tissue Lysate
MBS8415535-01mg	MyBiosource	0.1mg	1810 EUR
Human Liver Cirrhosis Muscle Diaphragm Tissue Lysate
MBS8415535-5x01mg	MyBiosource	5x0.1mg	7965 EUR
Brain (Stripped), Mature, Rabbit
MBS6120657-25Each	MyBiosource	25Each	670 EUR
Brain (Stripped), Mature, Rabbit
MBS6120657-5x25Each	MyBiosource	5x25Each	2785 EUR
Total RNA - Human Adult Normal Tissue: Skeletal Muscle
R1234171-50	Biochain	50 ug	221 EUR
Total RNA - Human Adult Normal Tissue: Muscle: Diaphragm
R1234169-10	Biochain	10 ug	221 EUR
Total RNA - Monkey (Rhesus) Normal Tissue: Skeletal Muscle
R1534171-50	Biochain	50 ug	229 EUR
Human Adult Skeletal Muscle (Normal) Whole tissue lysate
HAL-1375	Alpha Diagnostics	1 mg	628.8 EUR
Monkey (Cyno.) Normal Skeletal Muscle Whole tissue lysate
MCL-1197	Alpha Diagnostics	1 mg	628.8 EUR
Total Protein - Human Adult Normal Tissue: Skeletal Muscle
P1234171	Biochain	1 mg	216 EUR
Tissue, Total Protein, Mouse Adult Normal, Skeletal Muscle
MBS657016-1mg	MyBiosource	1mg	565 EUR
Tissue, Total Protein, Mouse Adult Normal, Skeletal Muscle
MBS657016-5x1mg	MyBiosource	5x1mg	2315 EUR
Tissue, Total Protein, Human Adult Normal, Skeletal Muscle
MBS657322-1mg	MyBiosource	1mg	565 EUR
Tissue, Total Protein, Human Adult Normal, Skeletal Muscle
MBS657322-5x1mg	MyBiosource	5x1mg	2315 EUR
Monkey (Rhesus) Normal Skeletal Muscle Whole tissue lysate
MRL-1247	Alpha Diagnostics	1 mg	628.8 EUR
Frozen Tissue Section - Human Adult Normal: Skeletal Muscle
T1234171	Biochain	5 slides	377 EUR
Tissue, Section, Human Adult Normal, Skeletal Muscle (Frozen)
MBS640631-5Sections	MyBiosource	5Sections	635 EUR
Tissue, Section, Human Adult Normal, Skeletal Muscle (Frozen)
MBS640631-5x5Sections	MyBiosource	5x5Sections	2640 EUR
Total RNA - Monkey (Cynomolgus) Normal Tissue: Skeletal Muscle
R1534171-Cy	Biochain	50 ug	229 EUR
Membrane Protein - Human Adult Normal Tissue: Skeletal Muscle
P3234171	Biochain	0.1 mg	311 EUR
Paraffin Tissue Section - Human Adult Normal: Skeletal Muscle
T2234171	Biochain	5 slides	204 EUR
Tissue, Section, Human Adult Normal, Skeletal Muscle (Paraffin)
MBS640137-5Sections	MyBiosource	5Sections	460 EUR
Tissue, Section, Human Adult Normal, Skeletal Muscle (Paraffin)
MBS640137-5x5Sections	MyBiosource	5x5Sections	1925 EUR
Tissue, Membrane Protein, Human Adult Normal, Skeletal Muscle
MBS639871-01mg	MyBiosource	0.1mg	570 EUR
Tissue, Membrane Protein, Human Adult Normal, Skeletal Muscle
MBS639871-5x01mg	MyBiosource	5x0.1mg	2345 EUR
Total RNA - Human Adult Normal Tissue: Heart: Papilla Muscle
R1234132-10	Biochain	10 ug	221 EUR
Tissue, Total Protein, Human Disease, Diabetes, Skeletal Muscle
MBS657193-1mg	MyBiosource	1mg	820 EUR
Tissue, Total Protein, Human Disease, Diabetes, Skeletal Muscle
MBS657193-5x1mg	MyBiosource	5x1mg	3470 EUR
Total Protein - Human Diabetic Diseased Tissue: Skeletal Muscle
P1236171Dia	Biochain	1 mg	542 EUR
Tissue, Section, Human Disease, Diabetes, Skeletal Muscle (Frozen)
MBS640417-5Slides	MyBiosource	5Slides	755 EUR
Tissue, Section, Human Disease, Diabetes, Skeletal Muscle (Frozen)
MBS640417-5x5Slides	MyBiosource	5x5Slides	3170 EUR
Rabbit SUBMAXILLARY MATURE 50 EA
41343-2	Pel-Freez	50 EA	382.07 EUR
Tissue, Membrane Protein, Human Disease, Diabetes, Skeletal Muscle
MBS639800-01mg	MyBiosource	0.1mg	695 EUR
Tissue, Membrane Protein, Human Disease, Diabetes, Skeletal Muscle
MBS639800-5x01mg	MyBiosource	5x0.1mg	2915 EUR
Tissue, Section, Human Disease, Diabetes, Skeletal Muscle (Paraffin)
MBS640643-5Slides	MyBiosource	5Slides	510 EUR
Tissue, Section, Human Disease, Diabetes, Skeletal Muscle (Paraffin)
MBS640643-5x5Slides	MyBiosource	5x5Slides	2145 EUR
Total Protein - Human Adult Normal Tissue: Heart: Papilla Muscle
P1234132	Biochain	1 mg	590 EUR
Tissue, Total Protein, Human Adult Normal, Heart, Papilla Muscle
MBS657154-1mg	MyBiosource	1mg	860 EUR
Tissue, Total Protein, Human Adult Normal, Heart, Papilla Muscle
MBS657154-5x1mg	MyBiosource	5x1mg	3640 EUR
Tissue, Total RNA, Rat Adult Normal, Skeletal Muscle, BioGenomics
MBS638559-005mg	MyBiosource	0.05mg	435 EUR
Tissue, Total RNA, Rat Adult Normal, Skeletal Muscle, BioGenomics
MBS638559-5x005mg	MyBiosource	5x0.05mg	1735 EUR
Human Soft Tissue Slide (Skeletal Muscle Normal) (5 slides/pk)
HTS-12341	Alpha Diagnostics	1 pk	343.2 EUR
Human Soft Tissue Slide (Skeletal Muscle Benign) (5 slides/pk)
HTS-12343	Alpha Diagnostics	1 pk	343.2 EUR
Skeletal Muscle Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack
TS-R5410	IHC World	-	145 EUR
Paraffin Tissue Section - Human Adult Normal: Heart: Papilla Muscle
T2234132	Biochain	5 slides	204 EUR
Tissue, Section, Human Adult Normal, Heart, Papilla Muscle (Paraffin)
MBS640350-5Sections	MyBiosource	5Sections	460 EUR
Tissue, Section, Human Adult Normal, Heart, Papilla Muscle (Paraffin)
MBS640350-5x5Sections	MyBiosource	5x5Sections	1925 EUR
Tissue, Total RNA, Mouse, Adult Normal, Skeletal Muscle, BioGenomics
MBS638546-005mg	MyBiosource	0.05mg	435 EUR
Tissue, Total RNA, Mouse, Adult Normal, Skeletal Muscle, BioGenomics
MBS638546-5x005mg	MyBiosource	5x0.05mg	1735 EUR
Tissue, Total RNA, Human Adult Normal, Skeletal Muscle, BioGenomics
MBS638548-005mg	MyBiosource	0.05mg	495 EUR
Tissue, Total RNA, Human Adult Normal, Skeletal Muscle, BioGenomics
MBS638548-5x005mg	MyBiosource	5x0.05mg	1995 EUR
Tissue, Genomic DNA, Rat Adult Normal, Skeletal Muscle, BioGenomics
MBS654525-01mg	MyBiosource	0.1mg	575 EUR
Tissue, Genomic DNA, Rat Adult Normal, Skeletal Muscle, BioGenomics
MBS654525-5x01mg	MyBiosource	5x0.1mg	2440 EUR
Tissue, Total RNA, Human Adult Normal, Muscle, Diaphragm, BioGenomics
MBS638673-001mg	MyBiosource	0.01mg	485 EUR
Tissue, Total RNA, Human Adult Normal, Muscle, Diaphragm, BioGenomics
MBS638673-5x001mg	MyBiosource	5x0.01mg	1960 EUR
Tissue cDNA, First Strand, Human Tumor, Skeletal Muscle, BioGenomics
MBS652570-10Tests	MyBiosource	10Tests	570 EUR
Tissue cDNA, First Strand, Human Tumor, Skeletal Muscle, BioGenomics
MBS652570-5x10Tests	MyBiosource	5x10Tests	2345 EUR
Human Soft Tissue Slide (Skeletal Muscle Malignant) (5 slides/pk)
HTS-12345	Alpha Diagnostics	1 pk	343.2 EUR
Skeletal Muscle Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack
TS-H5019	IHC World	-	145 EUR
Skeletal Muscle Tissue Slides, Normal Mouse Paraffin Sections, 5 slides/pack
TS-M5208	IHC World	-	145 EUR
Tissue, Genomic DNA, Human Adult Normal, Skeletal Muscle, BioGenomics
MBS654357-01mg	MyBiosource	0.1mg	575 EUR
Tissue, Genomic DNA, Human Adult Normal, Skeletal Muscle, BioGenomics
MBS654357-5x01mg	MyBiosource	5x0.1mg	2440 EUR
Tissue, Genomic DNA, Mouse Adult Normal, Skeletal Muscle, BioGenomics
MBS654457-01mg	MyBiosource	0.1mg	575 EUR
Tissue, Genomic DNA, Mouse Adult Normal, Skeletal Muscle, BioGenomics
MBS654457-5x01mg	MyBiosource	5x0.1mg	2440 EUR
Total RNA - Human Adult Normal Tissue 5 Donor Pool: Skeletal Muscle
R1234171-P	Biochain	50 ug	443 EUR
Rat Bladder Tissue Preparation Buffer 3: Normal Smooth Muscle Cells
9-80215	CHI Scientific	1 x 100 ml	Ask for price
Rat Rectal Tissue Preparation Buffer: Normal Rectal Smooth Muscle Cells
9-80288	CHI Scientific	1 x 100 ml	Ask for price
Tissue cDNA, First Strand, Rat Adult Normal, Skeletal Muscle, BioGenomics
MBS651951-40Tests	MyBiosource	40Tests	680 EUR
Tissue cDNA, First Strand, Rat Adult Normal, Skeletal Muscle, BioGenomics
MBS651951-5x40Tests	MyBiosource	5x40Tests	2835 EUR
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Skeletal Muscle
MBS640974-1Set	MyBiosource	1Set	650 EUR
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Skeletal Muscle
MBS640974-5x1Set	MyBiosource	5x1Set	2970 EUR
Human Rectal Tissue Preparation Buffer: Normal Rectal Smooth Muscle Cells
9-80100	CHI Scientific	1 x 100 ml	Ask for price
Mouse Rectal Tissue Preparation Buffer: Normal Rectal Smooth Muscle Cells
9-80198	CHI Scientific	1 x 100 ml	Ask for price
Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Skeletal Muscle, BioGenomics
MBS638519-005mg	MyBiosource	0.05mg	485 EUR

Inoltre, sono state generate due DP ciascuna in seguito all’interazione del farmaco e dell’acido tazarotenico con HCl, utilizzato come stressante acido. Questi sono stati provati sperimentalmente come pseudo DP, poiché non hanno avuto origine quando H2SO4 è stato impiegato come fattore di stress. Il farmaco era anche instabile in condizioni ossidative e fotolitiche, producendo sei DP. Tutti i prodotti sono stati separati su colonna a fase inversa (C18), utilizzando una fase mobile composta da formiato di ammonio 10 mM (pH 3.5) e acetonitrile, che è stata eseguita in modalità gradiente. I DP separati sono stati sottoposti a studi LC-HRMS e LC-MSn per la loro caratterizzazione iniziale.