Isotretinoina orale e uso topico di retinoidi in una serie di giovani pazienti con melanoma oculare

Scopo: descrivere la prima serie di sei pazienti giovani con melanoma uveale (UM) con isotretinoina orale e / o terapia topica con retinoidi prima della diagnosi.

Osservazioni: la serie di casi si basa su osservazioni cliniche presso il nostro centro di riferimento quaternario UM. Vengono segnalati sei casi di pazienti UM, di età compresa tra 16 e 44 anni. Tutti avevano usato un trattamento con retinoidi orale (isotretinoina) e / o topico (tretinoina o tazarotene) (3 mesi- ~ 10 anni) prima o al momento della diagnosi (3 su 6 casi). Tutti i pazienti presentavano disturbi oculari e l’insorgenza di alcuni sintomi corrispondeva al decorso dei retinoidi. Altri potenziali fattori di rischio o anamnesi rilevante includevano sfondo caucasico, distrofia conica e stato di fumatore attivo (caso 2), storia familiare di UM e gravidanza al momento della diagnosi (caso 3), fumo passato e possibile esposizione secondaria a Chernobyl da bambino ( Caso 5). Tutti i pazienti sono stati trattati con radioterapia a fascio di protoni e attualmente non hanno segni di malattia recidivante o metastatica.

Conclusioni e importanza: la terapia con retinoidi è stata collegata a vari effetti benigni e / o reversibili sull’occhio anteriore e posteriore, sebbene la fisiopatologia non sia ancora ben compresa. Il melanoma uveale (UM) è una rara diagnosi di cancro nei giovani adulti. Riportiamo qui la prima serie di casi di giovani pazienti di UM con una storia di uso di retinoidi e disturbi oculari. Non viene affermato alcun nesso causale e ulteriori studi epidemiologici e biologici sistematici sulla terapia con retinoidi e sull’impatto oculare possono fornire ulteriori dati rilevanti, in particolare nei pazienti giovani con melanoma oculare.

Aggiornamento teratogeno: uso topico e retinoidi di terza generazione

I primi retinoidi farmaceutici approvati dalla Food and Drug Administration degli Stati Uniti hanno ricevuto avvertimenti da scatola nera contro il loro uso in gravidanza a causa di potenziali effetti teratogeni. Questi retinoidi di prima e seconda generazione sono stati inizialmente formulati per il dosaggio orale e sono strutturalmente molto simili alla vitamina A, che ha effetti benefici sulla pelle e svolge un ruolo fondamentale nel guidare l’embriogenesi sana. Da allora, alcuni di questi retinoidi precoci sono stati riformulati per l’applicazione topica, il che ha ridotto il loro potenziale di esposizione sistemica. Inoltre, la progettazione razionale dei farmaci è stata applicata per creare i retinoidi di terza generazione di oggi (adapalene, tazarotene e bexarotene).

Questi composti includono anelli aromatici all’interno dei loro nuclei molecolari per fornire una rigidità strutturale che contrasta con la spina dorsale alifatica flessibile della vitamina A e delle precedenti generazioni di retinoidi, e quindi limita la loro attività fuori bersaglio. Come risultato di queste caratteristiche progettuali, il potenziale teratogeno negli animali dei retinoidi di terza generazione e in quelli riformulati per uso topico è generalmente inferiore a quello osservato con la somministrazione orale di generazioni precedenti di retinoidi. I dati epidemiologici disponibili, ma limitati, mostrano inoltre un potenziale teratogeno minimo o nullo associato all’uso nella vita reale di questi composti nell’uomo. Data la scarsità di dati epidemiologici disponibili in questo momento, tuttavia, si raccomanda di evitare l’uso di retinoidi topici durante la gravidanza. Tuttavia, in circostanze in cui può verificarsi un’esposizione involontaria in gravidanza, i dati disponibili forniscono una certa rassicurazione sul fatto che gli esiti avversi della gravidanza sono improbabili.

Chemerin come un driver di ipertensione: una considerazione

La proteina chemerina (gene indotto dal tazarotene, TIG2; RARRES2) è un’adipochina relativamente nuova. Molti studi supportano che i livelli di chemerina circolanti si associano fortemente e positivamente con BMI, grasso viscerale e pressione sanguigna. Qui, ci concentriamo sulla relazione specifica tra chemerina e pressione sanguigna con l’obiettivo di capire se e come la chemerina guida i cambiamenti (patologici) della pressione sanguigna in modo tale da poter essere interferiti terapeuticamente. Analizziamo la biosintesi della chemerina e come gli attuali farmaci antipertensivi modificano il metabolismo delle chemerine. Segue una rassegna di ciò che è noto su dove la chemerina è sintetizzata nel corpo e cosa possono fare la chemerina ei suoi recettori per la funzione fisiologica degli organi importanti per la determinazione della pressione sanguigna (ad es. Cervello, cuore, reni, vasi sanguigni, surrene e sistema nervoso simpatico).

Sintetizziamo dalla letteratura la nostra migliore comprensione dei meccanismi con cui la chemerina modifica la pressione sanguigna, con la consapevolezza che i livelli plasmatici / sierici di chemerina possono essere limitati nella loro rilevanza patologica. Questa revisione rivela diverse lacune nella nostra conoscenza della biologia delle chemerine che potrebbero essere colmate dal lavoro collettivo di chimici delle proteine, biologi, farmacologi e medici. Il tazarotene è un profarmaco che appartiene alla classe acetilenica dei retinoidi. Il farmaco è stato sottoposto a stress test idrolitico, ossidativo e fotolitico per stabilire la sua chimica di degradazione completa. Il farmaco si è dimostrato instabile in condizioni idrolitiche acide e basiche, producendo acido tazarotenico, che è un noto prodotto di degradazione principale (DP) e un metabolita attivo.

Soft Tissue Tissue Slide (Skeletal Muscle-Normal)
12-341-10um	ProSci	10 um	241.8 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-Normal)
12-341-4um	ProSci	4 um	216.6 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-Benign)
12-343-10um	ProSci	10 um	241.8 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-Benign)
12-343-4um	ProSci	4 um	216.6 EUR
Mouse Skeletal Muscle Tissue Lysate
LYSATE0021	BosterBio	200ug	180 EUR
Description: This cell lysate is prepared from mouse skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Soft Tissue Tissue Slide (Skeletal Muscle-malignant)
12-345-10um	ProSci	10 um	241.8 EUR
Soft Tissue Tissue Slide (Skeletal Muscle-malignant)
12-345-4um	ProSci	4 um	216.6 EUR
Rabbit OVARY MATURE 50 EA*
41329-2	Pel-Freez	50 EA	382.07 EUR
Rabbit ADRENAL MATURE 50 EA
41301-2	Pel-Freez	50 EA	409.48 EUR
Rabbit THYROID MATURE 50 EA*
41352-1	Pel-Freez	50 EA	366.41 EUR
Rabbit PANCREAS MATURE 25 EA*
41331-2	Pel-Freez	25 EA	409.48 EUR
Rabbit SUBMAXILLARY MATURE 50 EA
41343-2	Pel-Freez	50 EA	382.07 EUR
Rat Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells
9-80281	CHI Scientific	1 x 100 ml	Ask for price
Smooth muscle and striated muscle disease spectrum tissue array
SO8011	TissueArray	each	414 EUR
Description: Smooth muscle and striated muscle disease spectrum tissue array, including TNM and clinical stage, 80 cases /80 cores
Human Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells
9-80092	CHI Scientific	1 x 100 ml	Ask for price
Mouse Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells
9-80189	CHI Scientific	1 x 100 ml	Ask for price
Rat Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells
9-80280	CHI Scientific	1 x 100 ml	Ask for price
Human Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells
9-80091	CHI Scientific	1 x 100 ml	Ask for price
Mouse Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells
9-80188	CHI Scientific	1 x 100 ml	Ask for price
cDNA - Rat Normal Tissue: Skeletal Muscle
C1434171	Biochain	40 reactions	385 EUR
cDNA - Dog Normal Tissue: Skeletal Muscle
C1734171	Biochain	40 reactions	385 EUR
Rabbit LUNG UNTRIMMED MATURE 20 PR
41324-2	Pel-Freez	20 PR	323.34 EUR
cDNA - Human Tumor Tissue: Skeletal Muscle
C1235171-10	Biochain	10 reactions	274 EUR
Rat Skeletal Muscle Whole tissue lysate
RAL-1467	Alpha Diagnostics	1 mg	628.8 EUR
Rhabdomyosarcoma with striated muscle tissue array
SO2082b	TissueArray	each	546 EUR
Description: Rhabdomyosarcoma with striated muscle tissue array, including TNM and clinical stage, 104 cases/208 cores, replacing SO2082a
Muscle disease spectrum (smooth muscle and striated muscle cancer progression) tissue array
SO2081	TissueArray	each	474 EUR
Description: Muscle disease spectrum (smooth muscle and striated muscle cancer progression) tissue array, 104 cases/208 cores
cDNA - Mouse Normal Tissue: Skeletal Muscle
C1334171	Biochain	40 reactions	385 EUR
Mouse Skeletal Muscle Whole tissue lysate
MAL-1407	Alpha Diagnostics	1 mg	628.8 EUR
Frozen Tissue Section - Human Diabetic Diseased Tissue: Skeletal Muscle
T1236171Dia	Biochain	5 slides	475 EUR
Paraffin Tissue Section - Human Diabetic Diseased Tissue: Skeletal Muscle
T2236171Dia	Biochain	5 slides	238 EUR
Total RNA - Rat Normal Tissue: Skeletal Muscle
R1434171-50	Biochain	50 ug	130 EUR
Rat Muscle OptiTDS1: Tissue Dissociation System
4-28280	CHI Scientific	1 Kit	Ask for price
Rat Muscle OptiTDS2: Tissue Dissociation System
4-28281	CHI Scientific	1 Kit	Ask for price
Genomic DNA - Rat Normal Tissue: Skeletal Muscle
D1434171	Biochain	100 ug	286 EUR
Total RNA - Mouse Normal Tissue: Skeletal Muscle
R1334171-50	Biochain	50 ug	130 EUR
Human Muscle OptiTDS1: Tissue Dissociation System
4-28091	CHI Scientific	1 Kit	Ask for price
Human Muscle OptiTDS2: Tissue Dissociation System
4-28092	CHI Scientific	1 Kit	Ask for price
Mouse Muscle OptiTDS1: Tissue Dissociation System
4-28188	CHI Scientific	1 Kit	Ask for price
Mouse Muscle OptiTDS2: Tissue Dissociation System
4-28189	CHI Scientific	1 Kit	Ask for price
cDNA - Human Adult Normal Tissue: Skeletal Muscle
C1234171	Biochain	40 reactions	385 EUR
Genomic DNA - Mouse Normal Tissue: Skeletal Muscle
D1334171	Biochain	100 ug	286 EUR
cDNA - Monkey (Rhesus) Normal Tissue: Skeletal Muscle
C1534171	Biochain	40 reactions	385 EUR
Total Protein - Human Tumor Tissue: Skeletal Muscle
P1235171	Biochain	1 mg	310 EUR
Total Protein - Mouse Normal Tissue: Skeletal Muscle
P1334171	Biochain	1 mg	188 EUR
cDNA - Human Diabetic Diseased Tissue: Skeletal Muscle
C1236171Dia	Biochain	40 reactions	729 EUR
cDNA - Monkey (Cynomolgus) Normal Tissue: Skeletal Muscle
C1534171-Cy	Biochain	40 reactions	385 EUR
Total RNA - Human Adult Normal Tissue: Skeletal Muscle
R1234171-50	Biochain	50 ug	170 EUR
Total RNA - Human Adult Normal Tissue: Muscle: Diaphragm
R1234169-10	Biochain	10 ug	183 EUR
Total RNA - Monkey (Rhesus) Normal Tissue: Skeletal Muscle
R1534171-50	Biochain	50 ug	191 EUR
Monkey (Cyno.) Normal Skeletal Muscle Whole tissue lysate
MCL-1197	Alpha Diagnostics	1 mg	628.8 EUR
Human Adult Skeletal Muscle (Normal) Whole tissue lysate
HAL-1375	Alpha Diagnostics	1 mg	628.8 EUR
Total Protein - Human Adult Normal Tissue: Skeletal Muscle
P1234171	Biochain	1 mg	188 EUR
Monkey (Rhesus) Normal Skeletal Muscle Whole tissue lysate
MRL-1247	Alpha Diagnostics	1 mg	628.8 EUR
Frozen Tissue Section - Human Adult Normal: Skeletal Muscle
T1234171	Biochain	5 slides	343 EUR
Total RNA - Monkey (Cynomolgus) Normal Tissue: Skeletal Muscle
R1534171-Cy	Biochain	50 ug	191 EUR
Total RNA - Human Adult Normal Tissue: Heart: Papilla Muscle
R1234132-10	Biochain	10 ug	183 EUR
Membrane Protein - Human Adult Normal Tissue: Skeletal Muscle
P3234171	Biochain	0.1 mg	270 EUR
Paraffin Tissue Section - Human Adult Normal: Skeletal Muscle
T2234171	Biochain	5 slides	185 EUR
Total Protein - Human Diabetic Diseased Tissue: Skeletal Muscle
P1236171Dia	Biochain	1 mg	471 EUR
Total Protein - Human Adult Normal Tissue: Heart: Papilla Muscle
P1234132	Biochain	1 mg	513 EUR
Actin, Rabbit Muscle
P1246-1000	Biovision	each	274.8 EUR
Actin, Rabbit Muscle
P1246-500	Biovision	each	196.8 EUR
Human Soft Tissue Slide (Skeletal Muscle Normal) (5 slides/pk)
HTS-12341	Alpha Diagnostics	1 pk	343.2 EUR
Human Soft Tissue Slide (Skeletal Muscle Benign) (5 slides/pk)
HTS-12343	Alpha Diagnostics	1 pk	343.2 EUR
Paraffin Tissue Section - Human Adult Normal: Heart: Papilla Muscle
T2234132	Biochain	5 slides	185 EUR
Human Soft Tissue Slide (Skeletal Muscle Malignant) (5 slides/pk)
HTS-12345	Alpha Diagnostics	1 pk	343.2 EUR
Total RNA - Human Adult Normal Tissue 5 Donor Pool: Skeletal Muscle
R1234171-P	Biochain	50 ug	370 EUR
Rat Bladder Tissue Preparation Buffer 3: Normal Smooth Muscle Cells
9-80215	CHI Scientific	1 x 100 ml	Ask for price
Rat Rectal Tissue Preparation Buffer: Normal Rectal Smooth Muscle Cells
9-80288	CHI Scientific	1 x 100 ml	Ask for price
Human Rectal Tissue Preparation Buffer: Normal Rectal Smooth Muscle Cells
9-80100	CHI Scientific	1 x 100 ml	Ask for price
Mouse Rectal Tissue Preparation Buffer: Normal Rectal Smooth Muscle Cells
9-80198	CHI Scientific	1 x 100 ml	Ask for price
Rat Heart Tissue Preparation Buffer 1: Normal Aortic Smooth Muscle Cells
9-80253	CHI Scientific	1 x 100 ml	Ask for price
Aldolase (Rabbit Muscle) Antibody
abx022821-50mg	Abbexa	50 mg	1262.4 EUR
Human Heart Tissue Preparation Buffer 1: Normal Aortic Smooth Muscle Cells
9-80066	CHI Scientific	1 x 100 ml	Ask for price
Mouse Heart Tissue Preparation Buffer 1: Normal Aortic Smooth Muscle Cells
9-80159	CHI Scientific	1 x 100 ml	Ask for price
Rat Cervix Tissue Preparation Buffer 3: Normal Cervical Smooth Muscle Cells
9-80233	CHI Scientific	1 x 100 ml	Ask for price
Genomic DNA - Human Adult Normal Tissue: Skeletal Muscle, from a single donor
D1234171	Biochain	100 ug	286 EUR
Human Bladder Tissue Preparation Buffer 3: Normal Bladder Smooth Muscle Cells
9-80009	CHI Scientific	1 x 100 ml	Ask for price
Human Cervix Tissue Preparation Buffer 3: Normal Cervical Smooth Muscle Cells
9-80046	CHI Scientific	1 x 100 ml	Ask for price
Mouse Cervix Tissue Preparation Buffer 3: Normal Cervical Smooth Muscle Cells
9-80139	CHI Scientific	1 x 100 ml	Ask for price
Rat Colon Tissue Preparation Buffer 2: Normal Colorectal Smooth Muscle Cells
9-80237	CHI Scientific	1 x 100 ml	Ask for price
Rabbit MUSCLE ACETONE POWDER 10g
41995-2	Pel-Freez	10g	328.56 EUR
Purified (>95%) muscle Actin Protein from muscle (rabbit/bovine)
ACTB15-N-1	Alpha Diagnostics	1 mg	489.6 EUR
Rat Esophagus Tissue Preparation Buffer 2: Normal Esophageal Smooth Muscle Cells
9-80244	CHI Scientific	1 x 100 ml	Ask for price
Rat Intestine Tissue Preparation Buffer 2: Normal Intestinal Smooth Muscle Cells
9-80260	CHI Scientific	1 x 100 ml	Ask for price
Rat Lung Tissue Preparation Buffer 6: Normal Pulmonary Vein Smooth Muscle Cells
9-80276	CHI Scientific	1 x 100 ml	Ask for price
Genomic DNA - Human Diabetic Diseased Tissue: Skeletal Muscle, from a single donor
D1236171Dia	Biochain	50 ug	489 EUR
Actin, pyrene labeled, Rabbit Muscle
M1319-1000	Biovision	each	222 EUR
Actin, pyrene labeled, Rabbit Muscle
M1319-500	Biovision	each	183.6 EUR
Rabbit Anti Human Carboxypeptidase H (Mature N-Terminal) Polyclonal Antibody
CPBT-66584RH	Creative Diagnostics	0.1 ml	920.4 EUR
Human Esophagus Tissue Preparation Buffer 2: Normal Esophageal Smooth Muscle Cells
9-80056	CHI Scientific	1 x 100 ml	Ask for price
Human Intestine Tissue Preparation Buffer 2: Normal Intestinal Smooth Muscle Cells
9-80072	CHI Scientific	1 x 100 ml	Ask for price
Mouse Esophagus Tissue Preparation Buffer 2: Normal Esophageal Smooth Muscle Cells
9-80150	CHI Scientific	1 x 100 ml	Ask for price
Mouse Intestine Tissue Preparation Buffer 4: Normal Intestinal Smooth Muscle Cells
9-80168	CHI Scientific	1 x 100 ml	Ask for price
Human Lung Tissue Preparation Buffer 6: Normal Pulmonary Vein Smooth Muscle Cells
9-80086	CHI Scientific	1 x 100 ml	Ask for price
Mouse Lung Tissue Preparation Buffer 7: Normal Pulmonary Vein Smooth Muscle Cells
9-80184	CHI Scientific	1 x 100 ml	Ask for price
Rat Lung Tissue Preparation Buffer 4: Normal Pulmonary Artery Smooth Muscle Cells
9-80274	CHI Scientific	1 x 100 ml	Ask for price
Human Lung Tissue Preparation Buffer 4: Normal Pulmonary Artery Smooth Muscle Cells
9-80084	CHI Scientific	1 x 100 ml	Ask for price
Mouse Lung Tissue Preparation Buffer 5: Normal Pulmonary Artery Smooth Muscle Cells
9-80182	CHI Scientific	1 x 100 ml	Ask for price
Rabbit MUSCLE ACETONE POWDER (1-g)
41995-1	Pel-Freez	1g	144.55 EUR
Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80294	CHI Scientific	1 x 100 ml	Ask for price
Creatine Kinase (CK/CPK), Rabbit Muscle
P1301-1	Biovision	each	164.4 EUR
Creatine Kinase (CK/CPK), Rabbit Muscle
P1301-25	Biovision	each	679.2 EUR
alpha Actin (Skeletal Muscle) (Rabbit)
30R-3107	Fitzgerald	1 mg	308.4 EUR
Description: Purified native alpha Actin (Skeletal Muscle) (Rabbit)
Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80205	CHI Scientific	1 x 100 ml	Ask for price
Rabbit Skeletal Muscle Troponin-C
7-06205	CHI Scientific	1mg	Ask for price
Rabbit Skeletal Muscle Troponin-C
7-06206	CHI Scientific	5mg	Ask for price
Rabbit Skeletal Muscle Troponin-C
7-06207	CHI Scientific	10mg	Ask for price
Tissue Factor Rabbit mAb
A4395-100ul	Abclonal	100 ul	492 EUR
Tissue Factor Rabbit mAb
A4395-200ul	Abclonal	200 ul	685.2 EUR
Tissue Factor Rabbit mAb
A4395-20ul	Abclonal	20 ul	265.2 EUR
Tissue Factor Rabbit mAb
A4395-50ul	Abclonal	50 ul	344.4 EUR
Rat Airway Tissue Preparation Buffer 2: Normal Bronchial and Tracheal Smooth Muscle Cells
9-80209	CHI Scientific	1 x 100 ml	Ask for price
Rabbit Cofilin 2, Muscle ELISA kit
E04C0470-192T	BlueGene	192 tests	1524 EUR
Description: A competitive ELISA for quantitative measurement of Rabbit Cofilin 2, Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Cofilin 2, Muscle ELISA kit
E04C0470-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A competitive ELISA for quantitative measurement of Rabbit Cofilin 2, Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Cofilin 2, Muscle ELISA kit
E04C0470-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A competitive ELISA for quantitative measurement of Rabbit Cofilin 2, Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Liver Tissue Lysate
30R-AL003	Fitzgerald	150 ug	302.4 EUR
Description: Fresh tissue lysate prepared from rabbit liver
Human Airway Tissue Preparation Buffer 3: Normal Bronchial and Tracheal Smooth Muscle Cells
9-80003	CHI Scientific	1 x 100 ml	Ask for price
Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells
9-80110	CHI Scientific	1 x 100 ml	Ask for price
Mouse Airway Tissue Preparation Buffer 2: Normal Bronchial and Tracheal Smooth Muscle Cells
9-80116	CHI Scientific	1 x 100 ml	Ask for price
Rat Brain Tissue Preparation Buffer 11: Normal Cerebral Artery Vascular Smooth Muscle Cells
9-80220	CHI Scientific	1 x 100 ml	Ask for price
Rat Brain Tissue Preparation Buffer 5: Normal Cerebral Venous Vascular Smooth Muscle Cells
9-80223	CHI Scientific	1 x 100 ml	Ask for price
alpha smooth muscle actin Rabbit pAb
A0957-100ul	Abclonal	100 ul	460.8 EUR
alpha smooth muscle actin Rabbit pAb
A0957-200ul	Abclonal	200 ul	Ask for price
alpha smooth muscle actin Rabbit pAb
A0957-20ul	Abclonal	20 ul	Ask for price
alpha smooth muscle actin Rabbit pAb
A0957-50ul	Abclonal	50 ul	318 EUR
alpha smooth muscle actin Rabbit pAb
A1011-100ul	Abclonal	100 ul	369.6 EUR
alpha smooth muscle actin Rabbit pAb
A1011-200ul	Abclonal	200 ul	550.8 EUR
alpha smooth muscle actin Rabbit pAb
A1011-20ul	Abclonal	20 ul	219.6 EUR
alpha smooth muscle actin Rabbit pAb
A1011-50ul	Abclonal	50 ul	267.6 EUR
alpha smooth muscle Actin Rabbit mAb
A17910	Abclonal	20 μL	18 EUR
alpha smooth muscle Actin Rabbit mAb
A17910-100ul	Abclonal	100 ul	492 EUR
alpha smooth muscle Actin Rabbit mAb
A17910-200ul	Abclonal	200 ul	685.2 EUR
alpha smooth muscle Actin Rabbit mAb
A17910-20ul	Abclonal	20 ul	265.2 EUR
alpha smooth muscle Actin Rabbit mAb
A17910-50ul	Abclonal	50 ul	344.4 EUR
alpha smooth muscle actin Rabbit pAb
A7248-100ul	Abclonal	100 ul	369.6 EUR
alpha smooth muscle actin Rabbit pAb
A7248-200ul	Abclonal	200 ul	550.8 EUR
alpha smooth muscle actin Rabbit pAb
A7248-20ul	Abclonal	20 ul	219.6 EUR
alpha smooth muscle actin Rabbit pAb
A7248-50ul	Abclonal	50 ul	267.6 EUR
Rabbit Eye muscle antibody ELISA kit
E04E0148-192T	BlueGene	192 tests	1524 EUR
Description: A competitive ELISA for quantitative measurement of Rabbit Eye muscle antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Eye muscle antibody ELISA kit
E04E0148-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A competitive ELISA for quantitative measurement of Rabbit Eye muscle antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Eye muscle antibody ELISA kit
E04E0148-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A competitive ELISA for quantitative measurement of Rabbit Eye muscle antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit PSOAS MUSCLE YG FRESH 1 EA
41217-2CC	Pel-Freez	1 Each	234.6 EUR
Human Brain Tissue Preparation Buffer 3: Normal Cerebral Artery Vascular Smooth Muscle Cells
9-80015	CHI Scientific	1 x 100 ml	Ask for price
Human Brain Tissue Preparation Buffer 6: Normal Cerebral Venous Vascular Smooth Muscle Cells
9-80018	CHI Scientific	1 x 100 ml	Ask for price

Inoltre, sono state generate due DP ciascuna in seguito all’interazione del farmaco e dell’acido tazarotenico con HCl, utilizzato come stressante acido. Questi sono stati provati sperimentalmente come pseudo DP, poiché non hanno avuto origine quando H2SO4 è stato impiegato come fattore di stress. Il farmaco era anche instabile in condizioni ossidative e fotolitiche, producendo sei DP. Tutti i prodotti sono stati separati su colonna a fase inversa (C18), utilizzando una fase mobile composta da formiato di ammonio 10 mM (pH 3.5) e acetonitrile, che è stata eseguita in modalità gradiente. I DP separati sono stati sottoposti a studi LC-HRMS e LC-MSn per la loro caratterizzazione iniziale.