SFONDO
Il gene 1 indotto da tazarotene (TIG1) è un gene soppressore tumorale di tipo II inducibile dai retinoidi. L’isoforma B di TIG1 (TIG1B) inibisce la crescita e l’invasione delle cellule tumorali. L’espressione di TIG1B è spesso sottoregolata in vari tessuti tumorali; tuttavia, l’espressione e le attività dell’isoforma TIG1A devono ancora essere riportate. Pertanto, questo studio ha esaminato gli effetti delle isoforme TIG1A e TIG1B sulla crescita cellulare e sui profili di espressione genica utilizzando cellule di cancro del colon.
METODI
I cloni stabili TIG1A e TIG1B derivati da cellule di cancro al colon HCT116 e SW620 sono stati stabiliti utilizzando il sistema GeneSwitch; L’espressione dell’isoforma TIG1 è stata indotta dal trattamento con mifepristone. La crescita cellulare è stata valutata utilizzando i test di proliferazione cellulare e formazione di colonie WST-1. L’interferenza dell’RNA è stata utilizzata per esaminare i cambiamenti di mediazione di TIG1 nella crescita cellulare. I profili di espressione genica sono stati determinati utilizzando microarray e convalidati utilizzando la reazione a catena della polimerasi in tempo reale e analisi Western blot.
RISULTATI
Entrambe le isoforme di TIG1 sono state espresse a livelli elevati nei tessuti normali della prostata e del colon e sono state sottoregolate nelle linee cellulari di cancro del colon. Entrambe le isoforme TIG1 hanno inibito in modo significativo la crescita di cellule HCT116 transitoriamente trasfettate ed esprimendo stabilmente cellule TIG1A e TIG1B HCT116 e SW620. L’espressione dei geni 129 e 55 è stata alterata all’induzione dell’espressione di TIG1A e TIG1B, rispettivamente, nelle cellule HCT116 che esprimono stabilmente. Dei geni analizzati, 23 e 6 geni erano rispettivamente sovraregolati e sottoregolati nelle cellule che esprimono TIG1A e TIG1B. La sovraregolazione della chinasi 5 del recettore accoppiato alla proteina G (GRK5) è stata confermata utilizzando la reazione a catena della polimerasi in tempo reale e analisi Western blot in entrambe le linee cellulari stabili TIG1. Il silenziamento dell’espressione di TIG1A o GRK5 ha ridotto significativamente la soppressione della crescita cellulare mediata da TIG1A.
CONCLUSIONI
L’espressione di entrambe le isoforme di TIG1 è stata osservata nei tessuti normali della prostata e del colon ed è stata sottoregolata nelle linee cellulari di cancro del colon. Entrambe le isoforme TIG1 hanno soppresso la crescita cellulare e stimolato l’espressione di GRK5 nelle cellule HCT116 e SW620. Il knockdown dell’espressione di GRK5 ha alleviato la soppressione della crescita indotta da TIG1A delle cellule HCT116, suggerendo che GRK5 media la soppressione della crescita cellulare da parte di TIG1A. Pertanto, TIG1 può partecipare alla sottoregolazione della segnalazione accoppiata alla proteina G sovraregolando l’espressione di GRK5.
Metodi spettrofotometrici che indicano la stabilità per la determinazione del tazarotene in presenza del suo prodotto di degradazione alcalino mediante tecniche spettrofotometriche derivate.
È stata studiata la stabilità del tazarotene (TZ) e sono stati utilizzati due metodi di indicazione della stabilità, vale a dire, il derivato primo e un metodo spettrofotometrico del rapporto dei derivati, per determinare il tazarotene in presenza del suo prodotto di degradazione alcalina (HD) utilizzando metanolo come solvente. È stata ottenuta una relazione lineare nell’intervallo 1-10 µg ml ¹ per entrambi i metodi.
Applicando i metodi proposti, è stato possibile determinare il tazarotene nella sua polvere pura da con precisione 99,35 ± 1,410 (n = 10) per il metodo della derivata prima e 99,45 ± 1,053 (n = 10) per il metodo del rapporto dei derivati. I metodi del derivato primo e del rapporto dei derivati sono stati utilizzati per l’analisi di miscele preparate in laboratorio contenenti diversi rapporti di tazarotene e del suo prodotto di degradazione ed erano validi in presenza rispettivamente fino al 70% e all’80% di prodotto di degradazione. I metodi proposti sono stati convalidati e trovati adatti come metodi di analisi indicanti la stabilità per il tazarotene in formulazioni farmaceutiche.
[Retinoidi: meccanismi d’azione].
I retinoidi, derivati della vitamina A, sono molecole naturali o sintetiche con effetti pleiotropici, che regolano il differenziamento, la proliferazione e l’apoptosi cellulare. Nella cellula bersaglio, i metaboliti naturali attivi acido retinoico (RA) e acido 9-cis-retinoico sono sintetizzati dal retinolo mediante un processo in due fasi con il metabolita intermedio retinaldeide. Nel 1987, l’identificazione dei recettori nucleari dell’acido retinoico che appartengono alla superfamiglia dei recettori nucleari ha portato a un progresso significativo nella comprensione del meccanismo d’azione dei retinoidi. Esistono due famiglie di recettori nucleari retinoidi (RNR), i recettori RA (RAR), il cui ligando naturale è RA, e i recettori X retinoidi (RXR), il cui ligando naturale è acido 9-cis-retinoico. Tra i retinoidi sintetici, isotretinoina, acitretina, tazarotene e adapalene sono ligandi del RAR, il bexarotene è il primo rexinoide (ligando dell’RXR), l’alitretinoina il primo panagonista (RAR + RXR).
Tissue Factor Recombinant Rabbit mAb |
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| BS45170 | Bioworld Biotech | 50 ul | 398 EUR |
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Description: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
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Tissue Factor Recombinant Rabbit mAb |
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| BS46451 | Bioworld Biotech | 50 ul | 398 EUR |
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Description: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
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Tissue Factor Recombinant Rabbit mAb |
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| BS48447 | Bioworld Biotech | 50 ul | 398 EUR |
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Description: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
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Tissue Factor Recombinant Rabbit mAb |
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| BS48470 | Bioworld Biotech | 50 ul | 398 EUR |
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Description: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
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Tissue factor Recombinant Rabbit mAb |
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| E47M52647 | EnoGene | 100ul | 495 EUR |
Recombinant Rabbit Tissue factor (F3), partial |
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| MBS7111604-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Rabbit Tissue factor pathway inhibitor |
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| MBS9420734-002mg | MyBiosource | 0.02mg | 460 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor |
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| MBS9420734-01mg | MyBiosource | 0.1mg | 795 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor |
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| MBS9420734-1mg | MyBiosource | 1mg | 2525 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor |
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| MBS9420734-5x1mg | MyBiosource | 5x1mg | 11205 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor |
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| MBS9422521-002mg | MyBiosource | 0.02mg | 505 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor |
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| MBS9422521-01mg | MyBiosource | 0.1mg | 895 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor |
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| MBS9422521-05mg | MyBiosource | 0.5mg | 1975 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor |
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| MBS9422521-5x05mg | MyBiosource | 5x0.5mg | 8735 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| CSB-BP023437RB | Cusabio | 118 mg | Ask for price |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| CSB-BP023437RB-20ug | Cusabio | 20ug | Ask for price |
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Description: 25-300aa |
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Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| CSB-EP023437RB | Cusabio | 2318 mg | Ask for price |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| CSB-EP023437RB-20ug | Cusabio | 20ug | Ask for price |
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Description: 25-300aa |
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Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) |
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| AP70839 | SAB | 1mg | 2826 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) |
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| AP72812 | SAB | each | Ask for price |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| CSB-YP023437RB | Cusabio | 6035 mg | Ask for price |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| CSB-YP023437RB-20ug | Cusabio | 20ug | Ask for price |
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Description: 25-300aa |
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Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| MBS956430-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | 475 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| MBS956430-002mgEColi | MyBiosource | 0.02mg(E-Coli) | 375 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| MBS956430-002mgMammalianCell | MyBiosource | 0.02mg(Mammalian-Cell) | 580 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| MBS956430-002mgYeast | MyBiosource | 0.02mg(Yeast) | 420 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| MBS956430-01mgEColi | MyBiosource | 0.1mg(E-Coli) | 635 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| RPC22103-100ug | Biomatik Corporation | 100ug | 721.6 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| RPC22103-1mg | Biomatik Corporation | 1mg | 2884.7 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| RPC22103-20ug | Biomatik Corporation | 20ug | 448.1 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| RPC26335-100ug | Biomatik Corporation | 100ug | 1463 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) |
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| RPC26335-20ug | Biomatik Corporation | 20ug | 580.8 EUR |
Tissue factor Recombinant Rabbit mAb (S-893-34) |
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| S0B0577 | Starter Biotechnology Co Ltd | 10 μL | Ask for price |
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Description: Host :Rabbit |
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Tissue Factor Pathway Inhibitor Recombinant Rabbit mAb |
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| BS46527 | Bioworld Biotech | 50 ul | 398 EUR |
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Description: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
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Tissue Factor Pathway Inhibitor Recombinant Rabbit mAb |
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| BS46979 | Bioworld Biotech | 50 ul | 398 EUR |
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Description: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
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Tissue Factor Protein, Rabbit, Recombinant (ECD, His Tag) |
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| MBS8123288-002mg | MyBiosource | 0.02mg | 235 EUR |
Tissue Factor Protein, Rabbit, Recombinant (ECD, His Tag) |
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| MBS8123288-5x002mg | MyBiosource | 5x0.02mg | 915 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) (Active) |
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| CSB-MP023437RB | Cusabio | 9137 mg | Ask for price |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) (Active) |
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| CSB-MP023437RB-20ug | Cusabio | 20ug | Ask for price |
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Description: 25-300aa |
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Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) (Active) |
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| RPC26427-100ug | Biomatik Corporation | 100ug | 543.9 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) (Active) |
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| RPC26427-20ug | Biomatik Corporation | 20ug | 381.6 EUR |
OPCA00238-1MG - Recombinant Rabbit Tissue factor pathway inhibitor |
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| OPCA00238-1MG | Aviva Systems Biology | 1mg | 1977 EUR |
OPCA00238-100UG - Recombinant Rabbit Tissue factor pathway inhibitor |
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| OPCA00238-100UG | Aviva Systems Biology | 100ug | 586 EUR |
OPCA00238-20UG - Recombinant Rabbit Tissue factor pathway inhibitor |
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| OPCA00238-20UG | Aviva Systems Biology | 20ug | 400 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, E.coli-10ug |
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| QP6775-ec-10ug | EnQuireBio | 10ug | 391.2 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, E.coli-1mg |
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| QP6775-ec-1mg | EnQuireBio | 1mg | 2763.6 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, E.coli-50ug |
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| QP6775-ec-50ug | EnQuireBio | 50ug | 522 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, Yeast-1mg |
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| QP6775-ye-1mg | EnQuireBio | 1mg | 3296.4 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, E.coli-100ug |
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| QP6775-ec-100ug | EnQuireBio | 100ug | 848.4 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, E.coli-200ug |
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| QP6775-ec-200ug | EnQuireBio | 200ug | 1338 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, E.coli-500ug |
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| QP6775-ec-500ug | EnQuireBio | 500ug | 1816.8 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, Yeast-100ug |
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| QP6775-ye-100ug | EnQuireBio | 100ug | 945.6 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, Yeast-10ug |
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| QP6775-ye-10ug | EnQuireBio | 10ug | 434.4 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, Yeast-200ug |
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| QP6775-ye-200ug | EnQuireBio | 200ug | 1512 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, Yeast-500ug |
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| QP6775-ye-500ug | EnQuireBio | 500ug | 2164.8 EUR |
Recombinant Rabbit Tissue factor pathway inhibitor Protein, His, Yeast-50ug |
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| QP6775-ye-50ug | EnQuireBio | 50ug | 576 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2011301-001mg | MyBiosource | 0.01mg | 170 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2011301-005mg | MyBiosource | 0.05mg | 295 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2011301-01mg | MyBiosource | 0.1mg | 445 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2011301-02mg | MyBiosource | 0.2mg | 540 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2011301-05mg | MyBiosource | 0.5mg | 1025 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2012021-001mg | MyBiosource | 0.01mg | 175 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2012021-005mg | MyBiosource | 0.05mg | 315 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2012021-01mg | MyBiosource | 0.1mg | 480 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2012021-02mg | MyBiosource | 0.2mg | 585 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2012021-05mg | MyBiosource | 0.5mg | 1110 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2029924-001mg | MyBiosource | 0.01mg | 175 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2029924-005mg | MyBiosource | 0.05mg | 310 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2029924-01mg | MyBiosource | 0.1mg | 470 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2029924-02mg | MyBiosource | 0.2mg | 575 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2029924-05mg | MyBiosource | 0.5mg | 1090 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2010710-001mg | MyBiosource | 0.01mg | 175 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2010710-005mg | MyBiosource | 0.05mg | 310 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2010710-01mg | MyBiosource | 0.1mg | 470 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2010710-02mg | MyBiosource | 0.2mg | 575 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2010710-05mg | MyBiosource | 0.5mg | 1090 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2010916-001mg | MyBiosource | 0.01mg | 160 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2010916-005mg | MyBiosource | 0.05mg | 280 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2010916-01mg | MyBiosource | 0.1mg | 405 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2010916-02mg | MyBiosource | 0.2mg | 490 EUR |
Recombinant Tissue Factor (TF) |
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| MBS2010916-05mg | MyBiosource | 0.5mg | 930 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44024-100ug | Biomatik Corporation | 100ug | 554.4 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44024-1mg | Biomatik Corporation | 1mg | 2457 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44024-50ug | Biomatik Corporation | 50ug | 445.5 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44025-100ug | Biomatik Corporation | 100ug | 517 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44025-1mg | Biomatik Corporation | 1mg | 2293.2 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44025-50ug | Biomatik Corporation | 50ug | 415.8 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44026-100ug | Biomatik Corporation | 100ug | 566.5 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44026-1mg | Biomatik Corporation | 1mg | 2511.6 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44026-50ug | Biomatik Corporation | 50ug | 455.4 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44027-100ug | Biomatik Corporation | 100ug | 468.6 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44027-1mg | Biomatik Corporation | 1mg | 2074.8 EUR |
Recombinant Tissue Factor (TF) |
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| RPU44027-50ug | Biomatik Corporation | 50ug | 376.2 EUR |
Recombinant Tissue Factor (TF) |
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| RPU58458-100ug | Biomatik Corporation | 100ug | 551.1 EUR |
Recombinant Tissue Factor (TF) |
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| RPU58458-1mg | Biomatik Corporation | 1mg | 2558.4 EUR |
Recombinant Tissue Factor (TF) |
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| RPU58458-50ug | Biomatik Corporation | 50ug | 428.5 EUR |
Recombinant Tissue Factor (TF) |
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| RPU58988-100ug | Biomatik Corporation | 100ug | 947.4 EUR |
Recombinant Tissue Factor (TF) |
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| RPU58988-1mg | Biomatik Corporation | 1mg | 3731.5 EUR |
Recombinant Tissue Factor (TF) |
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| RPU58988-50ug | Biomatik Corporation | 50ug | 592 EUR |
Recombinant Tissue Factor (TF) |
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| RPU53495-100ug | Biomatik Corporation | 100ug | 554.4 EUR |
Recombinant Tissue Factor (TF) |
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| RPU53495-1mg | Biomatik Corporation | 1mg | 2457 EUR |
Recombinant Tissue Factor (TF) |
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| RPU53495-50ug | Biomatik Corporation | 50ug | 445.5 EUR |
Recombinant Tissue Factor (TF) |
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| RPA524Bo01 | Cloud-Clone | 10ug | 180 EUR |
Recombinant Tissue Factor (TF) |
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| 4-RPA524Bo01 | Cloud-Clone |
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Description: Recombinant Bovine Tissue Factor expressed in: E.coli |
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Recombinant Tissue Factor (TF) |
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| RPA524Hu01 | Cloud-Clone | 10ug | 180 EUR |
Recombinant Tissue Factor (TF) |
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| 4-RPA524Hu01 | Cloud-Clone |
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Description: Recombinant Human Tissue Factor expressed in: E.coli |
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Recombinant Tissue Factor (TF) |
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| RPA524Mu01 | Cloud-Clone | 10ug | 168 EUR |
Recombinant Tissue Factor (TF) |
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| 4-RPA524Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Tissue Factor expressed in: E.coli |
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Recombinant Tissue Factor (TF) |
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| RPA524Po01 | Cloud-Clone | 10ug | 164 EUR |
Recombinant Tissue Factor (TF) |
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| RPA524Ra01 | Cloud-Clone | 10ug | 184 EUR |
Recombinant Tissue Factor (TF) |
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| 4-RPA524Ra01 | Cloud-Clone |
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Description: Recombinant Rat Tissue Factor expressed in: E.coli |
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Recombinant Tissue Factor (TF) |
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| RPA524Rb01 | Cloud-Clone | 10ug | 152 EUR |
Recombinant Tissue Factor (TF) |
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| 4-RPA524Rb01 | Cloud-Clone |
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Description: Recombinant Rabbit Tissue Factor expressed in: E.coli |
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Tissue Factor Rabbit mAb |
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| A4395-100ul | Abclonal | 100 ul | 492 EUR |
Tissue Factor Rabbit mAb |
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| A4395-200ul | Abclonal | 200 ul | 685.2 EUR |
Tissue Factor Rabbit mAb |
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| A4395-20ul | Abclonal | 20 ul | 265.2 EUR |
Tissue Factor Rabbit mAb |
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| A4395-50ul | Abclonal | 50 ul | 344.4 EUR |
Tissue Factor Rabbit mAb |
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| 52668 | SAB | 100ul | 499 EUR |
Tissue Factor Rabbit mAb |
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| 59064 | SAB | 100ul | 339 EUR |
Tissue Factor Rabbit pAb |
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| E2381361 | EnoGene | 100ul | 225 EUR |
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Description: Available in various conjugation types. |
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Tissue Factor Rabbit mAb |
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| E2R26161 | EnoGene | 100ul | 255 EUR |
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Description: Available in various conjugation types. |
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Tissue Factor Rabbit mAb |
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| E2R27360 | EnoGene | 100ul | 255 EUR |
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Description: Available in various conjugation types. |
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Tissue Factor Rabbit mAb |
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| E2R27361 | EnoGene | 100ul | 255 EUR |
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Description: Available in various conjugation types. |
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Tissue Factor Rabbit mAb |
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| E2R381361 | EnoGene | 100ul | 275 EUR |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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Tissue factor Rabbit pAb |
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| E47R4690 | EnoGene | 100ul | 295 EUR |
Tissue Factor Rabbit mAb |
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| MBS4754094-01mL | MyBiosource | 0.1mL | 450 EUR |
Tissue Factor Rabbit mAb |
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| MBS4754094-5x01mL | MyBiosource | 5x0.1mL | 1540 EUR |
Tissue Factor Rabbit mAb |
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| MBS4754219-01mL | MyBiosource | 0.1mL | 450 EUR |
Tissue Factor Rabbit mAb |
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| MBS4754219-5x01mL | MyBiosource | 5x0.1mL | 1540 EUR |
Tissue Factor Rabbit mAb |
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| MBS4754220-01mL | MyBiosource | 0.1mL | 450 EUR |
Tissue Factor Rabbit mAb |
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| MBS4754220-5x01mL | MyBiosource | 5x0.1mL | 1540 EUR |
Tissue Factor Rabbit pAb |
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| MBS8542825-01mL | MyBiosource | 0.1mL | 305 EUR |
Tissue Factor Rabbit pAb |
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| MBS8542825-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
Tissue Factor Rabbit pAb |
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| MBS8542825-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
Tissue Factor Rabbit pAb |
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| MBS8542825-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
Tissue Factor Rabbit pAb |
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| MBS8542825-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
Tissue Factor Rabbit mAb |
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| MBS9467381-005mL | MyBiosource | 0.05mL | 290 EUR |
Tissue Factor Rabbit mAb |
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| MBS9467381-01mL | MyBiosource | 0.1mL | 435 EUR |
Tissue Factor Rabbit mAb |
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| MBS9467381-5x01mL | MyBiosource | 5x0.1mL | 1810 EUR |
Recombinant Human Tissue factor |
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| MBS7609429-005mg | MyBiosource | 0.05mg | 405 EUR |
Recombinant Human Tissue factor |
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| MBS7609429-02mg | MyBiosource | 0.2mg | 760 EUR |
Recombinant Human Tissue factor |
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| MBS7609429-1mg | MyBiosource | 1mg | 2175 EUR |
Recombinant Human Tissue factor |
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| MBS7609429-5x1mg | MyBiosource | 5x1mg | 8410 EUR |
Recombinant Human Tissue factor |
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| MBS967694-005mgBaculovirus | MyBiosource | 0.05mg(Baculovirus) | 1125 EUR |
Recombinant Human Tissue factor |
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| MBS967694-005mgEColi | MyBiosource | 0.05mg(E-Coli) | 260 EUR |
Recombinant Human Tissue factor |
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| MBS967694-02mgEColi | MyBiosource | 0.2mg(E-Coli) | 560 EUR |
Recombinant Human Tissue factor |
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| MBS967694-05mgEColi | MyBiosource | 0.5mg(E-Coli) | 875 EUR |
Recombinant Human Tissue factor |
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| MBS967694-1mgEColi | MyBiosource | 1mg(E-Coli) | 1325 EUR |
Tissue Factor Recombinant Protein |
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| 40-663 | ProSci | 2 ug | 311.1 EUR |
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Description: Tissue factor is a transmembrane glycoprotein of the cytokine receptor superfamily that acts as a receptor for coagulation factor VII (fVII) to trigger initiation of the coagulation cascade in response to vascular injury. Expression of tissue factor occurs constitutively within most extravascular and perivascular cells and at high levels within critical organs and tissue. Tissue factor is not normally expressed freely on the surface of circulating blood cells due to its pro-coagulant effect, but is instead stored on the surface of mononuclear and endothelial cells in microparticles that can shed into circulation in response to vascular injury, pro-inflammatory cytokines, or microbial ligands. Tissue factor can also be shed into circulation by cancer cells where its expression in a number of cancer types has been linked to tumor progression, metastatic potential, thrombosis, and angiogenesis. Expression of tissue factor has been shown to be inducible by select cytokines in a number of cell types, including IL-1β and TNF-α in vascular endothelial cells and macrophages, and TNF-α, IL-6, and FGF-Basic in monocytes, among others. CHO cell-derived Recombinant Human Tissue Factor is a glycoprotein homodimer comprised of the 214-amino-acid length monomer containing a C-terminal His-Tag. It has a calculated molecular weight of approximately 24.4 kDa, and as a result of glycosylation, Recombinant Human Tissue Factor migrates with an apparent molecular mass of approximately 45-55 kDa by SDS-PAGE gel, under reducing conditions. |
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Rabbit tissue factor X ELISA |
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| E01A34388 | BlueGene | 96T | 700 EUR |
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Description: ELISA |
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Rabbit tissue factor X ELISA |
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| E04T0135-48wellsplate | BlueGene | 48 wells plate | 280 EUR |
Rabbit tissue factor X ELISA |
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| E04T0135-96wellsplate | BlueGene | 96 wells plate | 405 EUR |
Rabbit Tissue Factor ELISA kit |
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| E01A34338 | BlueGene | 96T | 700 EUR |
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Description: ELISA |
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Rabbit tissue factor VII ELISA |
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| E01A34385 | BlueGene | 96T | 700 EUR |
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Description: ELISA |
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Rabbit Tissue Factor ELISA kit |
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| E04T0037-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Rabbit Tissue Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Tissue Factor ELISA kit |
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| E04T0037-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Rabbit Tissue Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Tissue Factor ELISA kit |
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| E04T0037-48wellsplate | BlueGene | 48 wells plate | 280 EUR |
Rabbit Tissue Factor ELISA kit |
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| E04T0037-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Rabbit Tissue Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Tissue Factor ELISA kit |
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| E04T0037-96wellsplate | BlueGene | 96 wells plate | 405 EUR |
Rabbit tissue factor VII ELISA |
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| E04T0131-48wellsplate | BlueGene | 48 wells plate | 280 EUR |
Rabbit tissue factor VII ELISA |
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| E04T0131-96wellsplate | BlueGene | 96 wells plate | 405 EUR |
Rabbit Tissue factor ELISA Kit |
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| ELA-E0524Rb | Lifescience Market | 96 Tests | 1113.6 EUR |
Rabbit Tissue Factor ELISA Kit |
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| MBS034222-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit Tissue Factor ELISA Kit |
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| MBS725252-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 5685 EUR |
Rabbit Tissue Factor ELISA Kit |
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| MBS725252-48StripWells | MyBiosource | 48-Strip-Wells | 485 EUR |
Per ogni famiglia ci sono 3 isotipi (α, β, γ) e per ogni isotipo diverse isoforme. Ogni NRR è composto da 6 regioni (A-F). 3 regioni sono importanti: la regione A / B ha una funzione di attivazione trascrizionale indipendente dal ligando, la regione C ospita il dominio di legame del DNA, la regione E ospita il dominio di legame del ligando. Per regolare l’espressione dei geni bersaglio, NRR deve dimerizzare. RXR sono obbligatori nei dimeri (eterodimeri RAR-RXR, omodimeri RXR-RXR). I dimeri si legano a specifiche sequenze di DNA, presenti nei promotori dei geni bersaglio. Quando il ligando, naturale o sintetico, si lega all’RNR, vengono reclutati i coattivatori e vengono attivati i fattori di trascrizione. Nella cellula bersaglio, i retinoidi non utilizzati vengono degradati nei metaboliti polari dagli enzimi del citocromo P450.
