Simian α2M(Alpha-2-Macroglobulin) ELISA Kit

Description: Our Human Alpha 2-Macroglobulin ELISA Kit is a sandwich ELISA for the detection of human alpha 2-macroglobulin in plasma, serum, lysates, and other biological fluids. Samples or standards are added to an anti-a2M antibody coated plate followed by the addition of a biotinylated anti-apoB-100 antibody. A streptavidin enzyme conjugate is then added for detection. The absorbance is read at 450nm and the level of a2M in samples is determined based on the provided human a2M standard curve.

Description: *Human Alpha-2 Macroglobulin ELISA Kit

Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rabbit;Sensitivity: 3.75 ng/ml

Description: Description of target: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. ;Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 2.330 ng/ml